LAB 9

                   MICROSCOPIC EXAMINATION OF HEART DEVELOPMENT                                  

 I. Introduction

Today’s lab will involve microscopic examination of the early development of gastrulation and neurulation in the frog and chick. As in the previous microscopic lab, this experience will allow us to perform experiments in vivo and prepare us for upcoming lab exam. You will be responsible for the following structures that can be found in the Mathews Lab manual:

 Frog:    Neural Fold and Neural Tube (Fig. 93-100)

4 mm Stage (Fig. 108-114) 

Neural Groove and Neural Tube

Notochord

Ectoderm, Mesoderm and Endoderm

Epidermis

Mesenchyme

Spinal Cord and Brain

Optic Vesicle and Lens Placode

Otic Vesicle

Heart

Adhesive Gland

Gut

 Chick:   18 hour WM (Fig. 154)

24 hour WM (Fig. 160) and XS (Fig. 163-167)

33 hr WM (Fig. 170) and XS (Fig. 174-181)

                        Primitive Groove, Streak and Knot

Notochord and Notochordal Process

Headfold

Somite and Segmental Mesoderm

Neural Fold and Neural Tube

Anterior Intestinal Portal

Head Ectoderm

Foregut and Midgut

Head Mesenchyme                                                                               

Endoderm

Lateral Plate Mesoderm

Optic Vesicles and Lens Placode

Heart and Dorsal Aorta

Otic Placode

Prosencephalon, Mesenchepalon and Rhombencephalon in 33 hour chick

You should also be able to tell the age of the embryo. For example a neural fold or a 4mm frog, or a 18 vs a 33 hour old chick. Most of the structures you will see are similar in most of the vertebrate embryos you will examine